Description
Test Principle: This assay employs a two-site sandwich ELISA to quantitate LAYN in samples. An antibody specific for LAYN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAYN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAYN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAYN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Layilin is a widely expressed integral membrane hyaluronan receptor, originally identified as a binding partner of talin located in membrane ruffles.An interdomain interaction between the amino- and the carboxy-terminal domains of radixin inhibits its ability to bind to layilin.In contrast, layilin binds both full-length and amino-terminal merlin-GST fusion proteins without a requirement for phospholipids. Furthermore, layilin antibody can immunoprecipitate merlin, confirming association in vivo between these two proteins, which also display similar subcellular localizations in ruffling membranes. Layilin may mediate signals from extracellular matrix to the cell cytoskeleton via interaction with different intracellular binding partners and thereby be involved in the modulation of cortical structures in the cell